General Cell Based Assays
ABclonal Technology CAMP Rabbit pAb
This gene encodes a member of an antimicrobial peptide family, characterized by a highly conserved N-terminal signal peptide containing a cathelin domain and a structurally variable cationic antimicrobial peptide, which is produced by extracellular proteolysis from the C-terminus. The protein plays an important role in innate immunity defense against viruses. In addition to its antibacterial, antifungal, and antiviral activities, the encoded protein functions in cell chemotaxis, immune mediator induction, and inflammatory response regulation.
American Research Products Inc Rat regulated on actiVation in
Rat regulated on actiVation in normal T-cell eXpressed and secreted/C-C motif chemokine 5 (RANTES/CCL5) ELISA kit from CUSABIO Detection Range 0 156 ng/mL-10 ng/mL Sensitivity 0 039 ng/mL Sample serum plasma cell culture supernates tissue homogenates Method Sandwich
Cayman Chemical TGR5GP-BAR1 Reporter asy 1ea
An IRE1a inhibitor (IC50s 0.2 and 0.02 UM for RNase and kinase activity respectively) selective for IRE1a over Ron and FGFR1 (IC50s 4.4 and 17 UM) as well as a panel of 282 kinases at 10 UM inhibits ER stress-induced increases in the splicing of mRNA encoding XBP1 in eight multiple myeloma cell lines at 8 UM reduces viral replication in tick-borne encephalitis virus-infected SH-SY5Y and SNB-19 cells at 0.5 UM and tick-borne encephalitis virus-infected Caco-2 cells at 1 UM
Bellbrook Labs Transcreener GDP FP Assay, Competitive Fluorescence Polarization Immunoassay, 10,000 assays, Far-red fluorescence, Homogeneous, mix-and-read format
Transcreener GDP FP Assay, Competitive Fluorescence Polarization Immunoassay, 10,000 assays, Far-red fluorescence, Homogeneous, mix-and-read format
Eurofins Discoverx PathHunter eXpress HTR2C (VNV) U2OS β-Arrestin GPCR Assay, 10pl Kit. (93-0938E3CP19L)
The PathHunter HTR2C(VNV) eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. serotonin) based activation of HTR2C(VNV) GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged HTR2C(VNV) and EA-tagged β-Arrestin-2. Activation of HTR2C(VNV) stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
ABclonal Technology ADAM17 Rabbit pAb
This gene encodes a member of the ADAM (a disintegrin and metalloprotease) family, which includes membrane-anchored proteins related to snake venom disintegrins. These proteins are involved in various biological processes such as fertilization, muscle development, and neurogenesis. The preproprotein is processed to produce the mature protease, which sheds the ectodomain of tumor necrosis factor-alpha (TNF-alpha), releasing soluble TNF-alpha. This protease also processes cell adhesion proteins, cytokine and growth factor receptors, and EGF receptor ligands, and activates the Notch signaling pathway. Elevated expression of this gene has been linked to psoriasis, rheumatoid arthritis, multiple sclerosis, and Crohn’s disease, suggesting a role in autoimmune diseases. Additionally, the protease may play a role in viral infection by cleaving ACE2, the receptor for SARS-CoV and SARS-CoV-2.
Enzo Life Sciences FLUOFORTE Calcium assay kit (96tests)
Fluo-Forte Calcium Assay Kits detect mobilization of intracellular calcium utilizing a fluorogenic calcium-binding dye optimized for superior cell-permeability and retention. The self-quenching dye undergoes an electronic change upon binding of calcium, resulting in a several order of magnitude greater fluorescence. Mechanism of Action The fluorogenic calcium-binding dye is provided to the cells as an acetoxymethyl (AM) ester, which is cell-permeable. Once inside the cells, the dye is hydrolyzed by intracellular esterases, which leads to generation of a cell membrane impermeable negatively charged form. In the absence of calcium, the calcium-binding moiety portion of the probe quenches the fluorescence of the fluorophore portion of the probe by photo-induced electron transfer. Applications: Flow Cytometry, Fluorescence microscopy, Fluorescent detection, HTS .
Biolegend Alexa FluorR 647 anti-AMPKalph
Alexa Fluor 647 anti-AMPKα Phospho (Thr172) [A20017A]; Isotype: Mouse IgG1, κ; Reactivity: Human, Mouse; Apps: IHC-P, ICFC; Size: 100 μg
Meso Scale Discovery U-PLEX Rat Leptin ASY 25 PL
The Meso Scale Discovery U-PLEX Rat Leptin Assay Kit is a multiplex immunoassay that measures leptin levels in rat serum plasma and cell culture supernatants The assay uses electrochemiluminescence detection technology and provides high sensitivity broad dynamic range and intra- and inter-assay precision This kit includes a 96-well plate coated with capture antibodies detection antibodies labeled with MSD SULFO-TAG calibrators and controls The assay has a lower limit of detection of 1 3 pg/mL and an upper limit of quantification of 10 000 pg/mL for leptin
Eurofins Discoverx PathHunter eXpress MC1R U2OS β-Arrestin GPCR Assay, 10-Plate Kit. (93-0861E3CP5L)
The PathHunter MC1R eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. α-MSH) based activation of MC1R GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged MC1R and EA-tagged β-Arrestin-2. Activation of MC1R stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress GHR-JAK1 Functional Assay, 10-Plate Kit. (93-0756E3CP0L)
The PathHunter GHR-JAK1 Functional assay eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting CTKs. This kit assesses GHR activation via detection of JAK1 mediated phosphorylation-induced SH2 domain protein recruitment. This product uses EFC technology. The included cell line overexpresses ProLink-tagged GHR cytokine receptor, an untagged CTK JAK1 and EA-tagged SH2 domain protein. Upon activation of the cytokine receptor, CTK-based receptor phosphorylation leads to SH2-EA recruitment and production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress GHR-JAK2 Functional Assay, 10-Plate Kit. (93-0829E3CP14L)
The PathHunter GHR-JAK2 Functional assay eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting CTKs. This kit assesses GHR activation via detection of JAK2 mediated phosphorylation-induced SH2 domain protein recruitment. This product uses EFC technology. The included cell line overexpresses ProLink-tagged GHR cytokine receptor, an untagged CTK JAK2 and EA-tagged SH2 domain protein. Upon activation of the cytokine receptor, CTK-based receptor phosphorylation leads to SH2-EA recruitment and production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress ACVR1C/ACVR2 Dimerization Assay, 2pl Kit. (93-1069E3CP5M)
The PathHunter ACVR1C/ACVR2 Dimerization eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting receptor-receptor interaction. This kit assesses ligand (e.g. activin B) induced dimerization of ACVR1C/ACVR2 receptors. This product uses EFC technology. The cell line included overexpresses ACVR1C target protein fused to PK alongside ACVR2 target protein fused to EA. Upon ligand-induced dimerization of ACVR1C/ACVR2, detectable EFC signal is produced. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.