General Cell Based Assays
Revvity Health Sciences Inc HTRF Human & Mouse Phospho-p70 S6K (Thr389) Detection Kit, Control Lysate
HTRF Human & Mouse Phospho-p70 S6K (Thr389) Detection Kit, Control Lysate
Revvity Health Sciences Inc HTRF Human & Mouse Total AMPK Detection Kit, 50,000 Assay Points
HTRF Human & Mouse Total AMPK Detection Kit, 50,000 Assay Points
Eurofins Discoverx cAMP Hunter eXpress SSTR3 CHO-K1 GPCR Assay, 2-Plate Kit. (95-0131E2CP2M)
The cAMP Hunter SSTR3 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. somatostatin 28) based activation of SSTR3 GPCR activity via detection of decreasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type SSTR3, a naturally Gi/Go coupled GPCR. Upon activation of SSTR3, the decrease in levels of intracellular cAMP leads to a decreased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter Human OXTR Activated GPCR Internalization Cell-Based Assay Kit (U2OS)
The PathHunter Human OXTR Stable Cell Line Activated GPCR Internalization Assay (U2OS) contains a stable clonal cell line used to measure OXTR (GPCR) internalization via early-endosome localization. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx PathHunter Human EphB4 Functional Kinase Cell-Based Assay Kit (U2OS)
The PathHunter Human EphB4 Stable Cell Line Functional Kinase Assay (U2OS) measures EphB4 receptor tyrosine kinase activity via ligand-induced SH2 domain protein recruitment. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx PathHunter Human SSTR4 β-Arrestin Cell-Based Assay Kit (CHO-K1)
The PathHunter Human SSTR4 β-Arrestin Stable Cell Line Assay (CHO-K1) contains a stable clonal cell line used to measure SSTR4 (GPCR) activity via recruitment of β-Arrestin2. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Cayman Chemical Annexin V FITC Assay Kit, 100 test
One of the hallmarks of the early stages of apoptosis is the redistribution of membrane phospholipids such as phosphatidylserine and phosphatidylethanolamine from the inner to outer leaflet of the membrane bilayer where they are exposed on the cell surface. Externalization of phosphatidylserine residues to the outer plasma membrane leaflet allows their detection via their high affinity for annexin V, a phospholipid binding protein. Cayman’s Annexin V Assay Kit employs FITC-conjugated Annexin V as a probe for phosphatidylserine on the outer membrane of apoptotic cells.
Eurofins Discoverx cAMP Hunter™ Human DRD2L Gi Cell-Based Assay Kit (CHO-K1)
The cAMP Hunter™ Human DRD2L Gi Stable Cell Line Assay (CHO-K1) contains a stable clonal cell line used to measure DRD2L (GPCR) activity via decreased levels of intracellular cAMP. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Sigma Aldrich Fine Chemicals Biosciences In Vitro Toxicology Assay
Tox7 is a member of Sigmas CytoToxicity Kit series. It is based on the measurement of lactate dehydrogenase (LDH) release from cells as a measure of cell membrane damage and cytotoxicity. This technique has been utilized as an alternative to 51Cr release for cell mediated cytotoxicity assays as well as conventional cytotoxicity resulting from interaction of a test material with the cell.
Eurofins Discoverx PathHunter eXpress BMPR1A/BMPR2 Dimerization Assay, 10pl Kit. (93-1006E3CP0L)
The PathHunter BMPR1A/BMPR2 Dimerization eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting receptor-receptor interaction. This kit assesses ligand (e.g. BMP-4) induced dimerization of BMPR1A/BMPR2 receptors. This product uses EFC technology. The cell line included overexpresses BMPR2 target protein fused to PK alongside BMPR1A target protein fused to EA. Upon ligand-induced dimerization of BMPR1A/BMPR2, detectable EFC signal is produced. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress P2RY2 Activated GPCR Internalization Assay, 2pl Kit. (93-0719E3CP5M)
The PathHunter P2RY2 Activated GPCR internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of P2RY2 via detection of localization to the early endosome. The included cell line overexpresses EA-tagged β-Arrestin-2 as well as a ProLink-tagged early endosome protein and untagged P2RY2. Upon ligand-induced receptor activation, β-Arrestin-2 is recruited to the activated GPCR and the resulting P2RY2-arrestin complex is internalized to the early endosome, leading to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx HITHUNTER CAMP ASSAY FOR SMALL
HitHunter cAMP Assay for Small Molecules, 2,500/10,000 dp (96-well/384-well).
Eurofins Discoverx PathHunter eXpress AGTRL1 CHO-K1 β-Arrestin GPCR Assay, 2-Plate Kit. (93-0250E2CP0M)
The PathHunter AGTRL1 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. apelin-13) based activation of AGTRL1 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged AGTRL1 and EA-tagged β-Arrestin-2. Activation of AGTRL1 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Sigma Aldrich Fine Chemicals Biosciences Af HDL and LDL/VLDL Assay
Cholesterol concentrations in High-Density Lipoprotein (HDL) and Low-Density (LDL)/Very-Low-Density (VLDL) Lipoproteins are strong predictors for coronary heart disease. functional HDL offers protection by removing cholesterol from cells and atheroma. Higher concentrations of LDL and lower concentrations of functional HDL are strongly associated with cardiovascular disease due to higher risk of atherosclerosis. The balances between high- and low-density lipoproteins are solely genetically determined but can be changed by medications food choices and other factors. Simple direct and automation-ready procedures for measuring HDL and LDL/VLDL concentrations are very desirable.