General Cell Based Assays
Cayman Chemical THUNDRPhosphop38a MAPK 2400Wel
Sandwich TR-FRET immunoassay for semi-quantitative detection of Phospho-p38αβγ MAPK14/11/12 (T180/Y182) in cell lysates
Cederlane L-Fucose Assay Kit
The L-Fucose test kit is a simple, rapid and reliable method, for the measurement and analysis of L-Fucose in plant extracts, biological samples and other materials. This kit can be used in the measurement of α-fucosidases that do not act on chromogenic substrates.
Apexbio Technology LLC LDH Cytotoxicity Assay Kit(Synonyms: LDH Cytotoxicity Detection Kit, LDH Release Assay Kit, Lactate Dehydrogenase Cytotoxicity Assay), 1000T.
The LDH Cytotoxicity Assay Kit is a biochemical assay designed to quantitatively assess cell cytotoxicity through measurement of lactate dehydrogenase (LDH) enzyme activity released into the cell culture medium LDH an intracellular enzyme ubiquitously expressed is released extracellularly upon damage or death of the cell membrane This assay relies on LDH-mediated conversion of lactate to pyruvate coupled with NAD reduction to NADH The reaction product NADH interacts with specific substrates within the kit generating a compound measurable spectrophotometrically at 490 nm Accordingly absorbance intensity at this wavelength directly correlates with cellular damage severity Compared to radioactive chromium ( 51Cr) release assays this method provides comparable quantitative data in a safer non-radioactive format applicable for cell death cytotoxicity and drug screening research
Eurofins Discoverx PathHunter eXpress HRH4 U2OS β-Arrestin GPCR Assay, 10-Plate Kit. (93-0209E3CP0L)
The PathHunter HRH4 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. histamine) based activation of HRH4 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged HRH4 and EA-tagged β-Arrestin-2. Activation of HRH4 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Meso Scale Discovery RPLEX HUM Influenza A NP ASY
The R-PLEX Human Influenza A NP Assay Kit from Meso Scale Diagnostics is a multiplex immunoassay that simultaneously detects influenza A nucleoprotein and differentiates between H1N1 and H3N2 subtypes in human nasopharyngeal swab specimens This assay utilizes MSD s electrochemiluminescent detection technology and provides results in under 5 hours It is intended for research use only and not for use in diagnostic procedures The kit includes a 96-well plate coated with capture antibodies detection antibodies read buffer wash buffer blocker solutions calibrators and controls
Meso Scale Discovery U-PLEX Mouse IL-5 ASY
The Meso Scale Discovery U-PLEX Mouse IL-5 Assay Kit is an electrochemiluminescent immunoassay for quantifying mouse interleukin 5 (IL-5) in serum plasma and cell culture supernatants It uses MSD s SECTOR Imager detecting IL-5 from 0 04 to 1000 pg/mL The kit includes a 96-well plate with capture antibody detection antibody with MSD SULFO-TAG calibrators and diluent The assay takes 3 5 hours and supports research on mouse models of asthma allergies and other IL-5-related immunological diseases
Meso Scale Discovery U-PLEX Mouse MIP-1 ASY
The Meso Scale Discovery U-PLEX Mouse MIP-1alpha Assay Kit is a multiplex immunoassay that quantitatively measures mouse MIP-1alpha in serum plasma and cell culture supernatants It utilizes electrochemiluminescence detection technology and provides high sensitivity up to 5 logs of dynamic range and low sample volume requirements The assay requires 50 L of sample and can be read on Meso Scale Discovery SECTOR Imagers The kit includes a plate coated with capture antibodies detection antibodies and calibrators ranging from 3 2 pg/mL to 100 000 pg/mL
Meso Scale Discovery U-PLEX Mouse IL-17A/F ASY
The Meso Scale Discovery U-PLEX Mouse IL-17A/F Assay is a multiplex immunoassay kit for quantifying mouse interleukin 17A and 17F in various biological matrices It uses electrochemiluminescence detection for high sensitivity and minimal cross-reactivity The assay requires 50 L or less sample volume and takes about 3 5 hours It includes a 96-well plate with capture antibodies detection antibodies with electrochemiluminescent labels calibrators and assay diluent enabling efficient and accurate measurement of IL-17A and IL-17F in mouse samples
Meso Scale Discovery R-PLEX Human ALK ASY
The R-PLEX Human ALK Assay QuickPlex 5-PL Kit from Meso Scale Diagnostics is a multiplex immunoassay for measuring ALK in human serum plasma and cell culture supernatants It uses electrochemiluminescence detection on the QuickPlex SQ 120 instrument The assay takes about 3 hours and needs 12 5 L of sample with a detection range of 19 to 10 000 pg/mL The kit includes reagents for calibration controls diluent detection antibodies read buffer and plates with capture antibodies
Cayman Chemical BIlIrubIn ConjugatesodIM100mg
A stable, water-soluble ditaurate derivative of bilirubin meant to mimic endogenous bilirubin glucuronide derivatives; exhibits anti-oxidant and antimutagenic effects
Meso Scale Discovery R-PLEX Human ICAM-3 ASY
The R-PLEX Human ICAM-3 Assay Kit from Meso Scale Discovery measures Intercellular Adhesion Molecule 3 (ICAM-3) in human serum plasma and cell culture supernatants This electrochemiluminescent immunoassay uses MSD s SULFO-TAG labels and multi-array technology for sensitive detection The kit includes a 96-well plate with capture antibody detection antibody with ruthenium label calibrators and necessary reagents Results are available in 3 5 hours with a dynamic range of 16-10 000 pg/mL offering a simple reproducible method to measure soluble ICAM-3 levels
Eurofins Discoverx PathHunter eXpress CCRL2 CHO-K1 β-Arrestin Orphan GPCR Assay, 10-Plate Kit. (93-0333E2ACP1L)
The PathHunter CCRL2 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. CCL19) based activation of CCRL2 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged CCRL2 and EA-tagged β-Arrestin-2. Activation of CCRL2 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress GPR12 CHO-K1 β-Arrestin Orphan GPCR Assay, 2-Plate Kit. (93-0329E2ACP1M)
The PathHunter GPR12 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand based activation of GPR12 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GPR12 and EA-tagged β-Arrestin-2. Activation of GPR12 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. This is an orphan receptor without a known ligand. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.